Department of Biology, Manado State University, Sulawesi Utara, Indonesia
Utari Satiman has extended valuable service for many years. Also has been a recipient of many awards and grants. International experience includes various programs, contributions and participation in different countries for diverse fields of study
Research Article
Identification by 16S Ribosomal RNA Gene Sequencing of Lactic Acid Bacteria Producing Antibacterial Agents from Langsat Fruit (Lansium domesticum) in North Minahasa District, North Sulawesi
Author(s): Helen Joan Lawalata*, Mariana Rengkuan and Utari Satiman
Fourty two strains Lactic Acid Bacteria (LAB) were isolated from Langsat fruit (Lansium domesticum) samples obtained from 4 districts in Minahasa, North Sulawesi. 12 isolates of lactic acid bacteria were isolated from Langsat fruit in North Minahasa District. Lactic acid bacteria have the ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The aim of this study was to identify LAB isolates producing antibacterial agents from Langsat fruit at North Minahasa District based on identification genotypic. Identification genotypic isolat LMU7 from Langsat fruit was based on 16S rRNA gene sequencing Method. One Isolate (LMU7) had a great ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The 16S ribosomal RNA gene from one isolate could be amplified by PCR (Polymerase Chain Reaction) and show a single band on a 2% (w/v) agarose gel. Ide.. View more»