Dr, Department of Biology, Faculty of Mathematics and Natural Science, Manado State University, Sulawesi Utara, Indonesia
Helen Joan Lawalata has extended his valuable service for many years and has been a recipient of many award and grants. His international experience includes various programs, contributions and participation in different countries for diverse fields of study. His research interests reflect in his wide range of publications in various national and international journals.
Research Article
Identification by 16S Ribosomal RNA Gene Sequencing of Lactic Acid Bacteria Producing Antibacterial Agents from Langsat Fruit (Lansium domesticum) in North Minahasa District, North Sulawesi
Author(s): Helen Joan Lawalata*, Mariana Rengkuan and Utari Satiman
Fourty two strains Lactic Acid Bacteria (LAB) were isolated from Langsat fruit (Lansium domesticum) samples obtained from 4 districts in Minahasa, North Sulawesi. 12 isolates of lactic acid bacteria were isolated from Langsat fruit in North Minahasa District. Lactic acid bacteria have the ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The aim of this study was to identify LAB isolates producing antibacterial agents from Langsat fruit at North Minahasa District based on identification genotypic. Identification genotypic isolat LMU7 from Langsat fruit was based on 16S rRNA gene sequencing Method. One Isolate (LMU7) had a great ability to inhibit the growth of pathogenic bacteria and putrefactive bacteria. The 16S ribosomal RNA gene from one isolate could be amplified by PCR (Polymerase Chain Reaction) and show a single band on a 2% (w/v) agarose gel. Ide.. View more»