Department of Chemical Engineering, College of Engineering and Technology, Wolkite University, Wolkite, Ethiopia
Research Article
Identification and Characterization of Caffeine Degrading Microbes from Coffee Husk for Production of Liquid Bio Fertilizer and Process Optimization by Using Rhizobium Bacteria
Author(s): Zeynu Shamil*
Bioremediation being a problem solver for many different environmental and other major hazards, they play an important and cost effective role in the decaffeination process. Within this experimental work eliminate caffeine from coffee husk then we take Rhizobium bacterial strain from Ethiopia bio diversity institute. We observe that could grow on the medium supplemented with 2.5g/L of caffeine i.e., could effectively degrade 2.5 g/L of caffeine in the liquid media as a sole source of carbon and nitrogen. Morphological and biochemical characteristics were maintained for re-affirmation the organism as Rhizobium . The degradation of caffeine capability was authenticated by growth curve. And secondly we observed that the potency of caffeine degradation caffeine degrading bacteria was maintained by standard medium with and without supplement of 2.5g/l of caffeine.. View more»