Journal of Clinical & Experimental Pharmacology

INSERM U1132 and University Paris 7, Hôspital Lariboisière, Paris, France

Publications

• Research Article   
  Evaluation of Intra-Articular Delivery Of The Internal Phase of A Sodium Hyaluronate and Indomethacin-Based Nanoemulsion in Primary Articular Chondrocytes
  Author(s): Ibtissema G*, Yohanb J, Ericb HAY, Carolineb M, Martineb CS and Souada S
  
  In this study, we aimed to investigate the internalisation of the internal phase of a sodium hyaluronate (HNa) and indomethacin (Indo)-based nanoemulsion (NE) in murine articular chondrocytes, cells that secrete cartilage matrix. Immunofluorescence with antibodies against collagen II (Col2a1) and aggrecan (Acan) was used to assess collagen type II and aggrecan expression. RNA was extracted for analysing the expression of (metalloprotease 3) Mmp3, (metalloprotease 13) Mmp13, Acan, Col2a1, and Sox9 using polymerase chain reaction (PCR). Hyalgan was used as reference and a solution of each of the active substances (HNa and Indo) were used as controls. The chondrocytes reached confluence in a few hours, and 1% of the internal phase of the nanoemulsion exhibited a chondroprotective effect. Immunofluorescence using anti-Col II antibody showed expression of collagen, whereas that with anti-a.. View more»
  

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