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Woubit Dawit
Forage genebank, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia
Publications
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Research Article
Reverse Transcriptase Loop-Mediated Isothermal Amplification Assays (RTLAMP) for the Detection of Alfalfa Mosaic Virus from Forage Crops
Author(s): Woubit Dawit*, Fikerte Mulatu, Yesuf Eshete and Alice Muchugi
AMV is one of the most economically important plant viruses and has a very wide host range including forage crops. To make sure that only seeds of satisfactory phytosanitary status are distributed to recipients in geographically diverse areas, the ILRI genebank routinely monitors seed-borne diseases during seed multiplication and in the field genebank. Current detection techniques for AMV include a dot-blot assay and a two-step Reverse Transcription Polymerase Chain Reaction (RT-PCR), both of which are time consuming. In the present study we developed a onestep Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) method for the sensitive and rapid detection of AMV from forage crops. For the assay, six different AMV specific primers were used, and a series of reactions were performed to identify optimal conditions. Amplicons were easily visualized by means of an in-tu.. View more»