Journal of Bioequivalence & Bioavailability

Department of Chemical and Biomolecular Engineering, National University of Singapore, Lower Kent Ridge, 119077, Singapore

Publications

• Research   
  Effect of Polyethylene Glycol on Growth of Escherichia coli DH5α and Bacillus subtilis NRS-762
  Author(s): Wenfa NG*
  
  Polyethylene glycol is commonly used in fermentation as anti-foam for preventing the rise of foam to the top plate of the bioreactor, which increases contamination risk. However, its potential toxicity to growth of various microorganisms is not well understood at the species and strain level. Hence, the objective of this study was to understand the impact of different concentrations of polyethylene glycol at the 1, 5 and 10 g/L level on the aerobic growth of Escherichia coli DH5α and Bacillus subtilis NRS-762 in LB Lennox medium in shake flasks. Experiment results revealed that polyethylene glycol (PEG) (molecular weight ~8000 Da), at all concentrations tested and did not affect biomass formation and metabolism in E. coli DH5α at 37ᵒC. This came about through the observation of similar maximal optical density obtained during growth of E. coli DH5.. View more»
  

  Abstract PDF