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Journal of Bioequivalence & Bioavailability

Wenfa NG

Department of Chemical and Biomolecular Engineering, National University of Singapore, Lower Kent Ridge, 119077, Singapore

Publications
  • Research   
    Drastic Decline in Optical Density during Stationary Phase Meant that Bacillus subtilis NRS-762 is not Suitable as Model Organism in Microbial Survivability Studies
    Author(s): Wenfa NG*

    Survival of microbes on various surfaces and habitats is a question of importance to basic science, as well as health care, water treatment and distribution, ecology, and search for life in other planetary bodies. To this end, various model organisms known to be resilient against a variety of environmental stressors are used for understanding the mechanisms underlying survival in extreme environments, or conditions mimicking those of the investigated habitats. Observations of drastic decline in optical density of Bacillus subtilis NRS-762 (ATCC 8473) in LB Lennox and Tryptic Soy Broth (TSB) at temperatures of 25°C, 30°C and 37°C, after the aerobic shake flask culture reached maximal cell density at stationary phase, pointed to possible cell lysis as mechanism for cell death. Specifically, optical density of the bacterium declined from 5.4 at 22.5 hours.. View more»

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