Al-Jouf University College of Applied Medical Sciences, Clinical Laboratory Sciences, Umar Ibn Abdulaziz, Al Jouf University, Kingdom of Saudi Arabia
Waleed Abu Al-Soud has extended valuable service for many years. Also has been a recipient of many awards and grants. International experience includes various programs, contributions and participation in different countries for diverse fields of study
Research Article
Detection of the Panton-Valentine Leukocidin Gene in Swedish Isolates of Methicillin-Resistant Staphylococcus aureus using a Multiplex PCR Assay
Author(s): Waleed Abu Al-Soud*
Background: Panton-Valentine leukocidin (PVL) is a bicomponent leukotoxin produced by <2% of Staphylococcus aureus (S. aureus) strains. PVL forms transmembrane pores that cause cell disruption and death. PVL is associated with skin necrotic lesions and severe necrotizing pneumonia. The methicillin-resistant S. aureus (MRSA) strains that harbor the lukS-PV gene (the gene encoding PVL toxin) are considered highly pathogenic since they can cause infections that are difficult to treat.
Objective: This study aimed to optimize a multiplex PCR assay that can detect both methicillin-resistant S. aureus strains and their ability to produce PVL toxin isolated from Swedish patients.
Methods: An optimized multiplex PCR-assay was developed to detect both nuc and lukS-PV genes and was appli.. View more»