Tigray Biotechnology Center, Mekelle, Ethiopia
Tsehaye Kidus has extended valuable service for many years. Also has been a recipient of many awards and grants. International experience includes various programs, contributions and participation in different countries for diverse fields of study
Research Article
Isolation, Characterization and Identification of Contaminant Bacteria from Sugarcane (Saccharum officinarum L.) in vitro Culture in Tigray Biotechnology Center, Mekelle, Ethiopia
Author(s): Tsehaye Kidus* and Zenebe Teka
Plant tissue cultures can be contaminated by extensive diversity of bacteria and the contaminations are species specific. The contamination can reduce growth rate of shoot and root, multiplication factor, and even cause plant death. The study was conducted at Mekelle University, College of Veterinary Medicine and Tigray Biotechnology center and contaminated in vitro sugarcane culture obtained from laboratory of plant tissue culture. The objective of the study was to isolate, characterize, and identify bacteria from contaminated in vitro Sugarcane culture and to test the sensitivity of the isolates to the most commonly used antibiotics. Morphological, Gram stain, Endospore stain and Biochemical test method were used to isolate and identify the contaminates and disk diffusion method was used for the drug susceptibility test. Commonly available antibiotics were used for.. View more»