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Siti Subandiyah
Department of Agriculture, Gadjah Mada University, Yogyakarta, Indonesia
Publications
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Short Communication
Selection of Rhizobacteria to Control Twisted Disease of Shallot
Author(s): Dina Istiqomah*, Ayu Lestiyani, Arif Wibowo, Tri Joko and Siti Subandiyah
The purpose of this study is to find out the type of antagonistic rhizobacteria and their capability in F. acutatum growth suppression on in vitro and in vivo condition. The study was conducted by selecting of rhizobacteria isolated from Shallot’s rhizosphere which was taken from 4 regions; they were Bantul district (Yogyakarta), Nganjuk (East Java), Cirebon (West Java) and Brebes (Central Java) district. Characterization was taken based on physiology and biochemist, PCR based on 16S rRNA and Rep-PCR (BOX-PCR and ERIC-PCR). The in vivo test was carried out by factorial experiment with 2 trials using completelly randomized design i.e., 1) soaking shallot seeds into rhizobacteria suspension for 1 hour before planting and 2) watering plants with rhizobacteria suspension in once 2 weeks. Observation variables consists of.. View more»