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Shifa Chaudhary
Shriram Institute for Industrial Research, Analytical Science Division, Delhi, India
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Research
The Potential Role of Plastome as a Biomarker and Biosensor Using Real- time Quantitative Polymerase Chain Reaction
Author(s): Amita Pandey*, Shifa Chaudhary and Binu Bhat
Plastids are semiautonomous eukaryotic organelles specific to plants performing many crucial and critical functions including photosynthesis, energy generation, development, stress perception, storage, flowering, and fruit ripening. Studies have shown that the number of chloroplast, a plastid subtype is affected by biotic and abiotic stresses and the Plastome Copy Number (PCN), circular DNA molecules present in the plastids are regulated by developmental stage and environmental stimuli. This study for the first time proposes the use of PCN as a biomarker and biosensor based on parsing real-time qPCR data obtained over a period of two years by using plastid tRNA specific primers from leaves and lint samples of cotton and seed samples of cotton, rice, soybean, maize, and sesame. PCN can be used because as opposed to nuclear DNA the PCN is modulated by both internal .. View more»