Centre for Applied Molecular Biology (CAMB), University of the Punjab, Lahore, Pakistan
Safdar Hussain has extended his valuable service for many years and has been a recipient of many award and grants. His international experience includes various programs, contributions and participation in different countries for diverse fields of study. His research interests reflect in his wide range of publications in various national and international journals.
Research Article
Molecular Characterization of Gut Microbiota in Obese and Lean Hypertensive Patients
Author(s): Nosheen Mushtaq, Safdar Hussain and Jiru Xu*
Hypertension (HTN) and obesity are the major risk factors for cardiovascular diseases, and the gut microbiota has emerged to play a role in the development of many of the diseases including hypertension. In this study we hypothesized that obesity in hypertension could be linked to a specific gut microbiota. Our aim was to quantify and evaluate the differences in the composition of gut microbiota between obese and lean hypertensive patients and compared them with healthy controls. Fecal samples from 30 obese and 30 lean hypertensive patients and 30 samples from healthy individuals were collected. The samples were analyzed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) using primers, specifically targeting the V3 region of the bacterial 16s ribosomal RNA gene. Absolute quantification of the bacterial species such as Prevotella spp., Bacteroides spp., Clostri.. View more»