Journal of Bioequivalence & Bioavailability

Basic & Pharmaceutical Sciences Division, College of Pharmacy & Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL, USA

Biography

Ronald D Thomas currently works in the Basic & Pharmaceutical Sciences Division, College of Pharmacy & Pharmaceutical Sciences, Florida A&M University, 2074 Midyette Rd, Suite 117, Tallahassee, FL, USA. His research interest includes DNA damage; Strand break

Publications

• Research Article   
  Crude Edible Fig (Ficus carica) Leaf Extract Prevents Diethylstilbestrol (DES)-Induced DNA Strand Breaks in Single-Cell Gel Electrophoresis (SCGE)/Comet Assay: Literature Review and Pilot Study
  Author(s): Alrena V Lightbourn* and Ronald D Thomas
  
  Fig (Ficus carica) trees are among the oldest plants on earth. The chemopreventive properties of constituent polyphenols and fiber that implicate figs in having a functional role in averting cancer have not been fully elucidated. We therefore hypothesized that fig leaf extract would inhibit (or attenuate) DES-induced DNA single-strand breakage in MCF10A human breast epithelial cells. To test this hypothesis, MCF10A cells were treated with DES (1, 10, 100 μM), crude fig leaf extract (5, 10, 15 μL), or concomitant doses of DES (100 μM)/fig leaf extract (5, 10, 15 μL). The cells were analyzed for DNA strand breakage using the SCGE/COMET assay with mean olive tail moment as a marker of DNA damage. DES induced DNA strand breaks at all treatment levels compared to DMSO and non-treatment controls. DES at concentrations of 1, 10, and 100 μM produced mean olive tail mom.. View more»
  

  DOI: 10.35248/0975-0851.19.11.389

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