Journal of Plant Pathology & Microbiology

Department of Plant Production, Soil Science and Agricultural Engineering, Green Biotechnologies Research Centre of Excellence, University of Limpopo, Sovenga, South Africa

Publications

• Research   
  Phenotypic and Phylogenetic Analyses of Residual Bacteria in Effective Microorganism-Fermented Cucurbitacin Phytonematicide
  Author(s): Phatu W. Mashela* and Kgabo M. Pofu
  
  Information on residual bacteria that keep cucurbitacin phytonematicides aseptic for extended periods is not available. At 35 weeks after initiating fermentation, samples were collected from Nemafric-BL phytonematicide, with the cultured strain subjected to various phenotypic and phylogenetic tests. Phenotypic tests characterized the test residual bacteria as Gram-positive Lactobacillus strain, with energy-efficient homolactic fermentative process, but without CO2 as an end-product. Phylogenetic analysis using ZymoBIOMICSTM DNA Miniprep Kit for Gram- positive bacteria, with subsequent PCR and GenBank analyses resulted in 16S rRNA gene sequences that clustered the residual Lactobacillus strain with the Spanish HG794492 (acc. no.) Lactobacillus strain, AY681132 Lactobacillus vini and AB242320 Lactobacillus mobilis, with 75% 16S rRNA gene sequence similarity... View more»
  

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