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Nilesh I. Patil
Department of Pharmacy, Shri Prakashchand Jain College of Pharmacy and Research, Palaskheda, India
Publications
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Research Article
Development and Validation of RP-HPLC Method for Estimation of Ramipril in Tablet Dosage Form
Author(s): Prashant P. Nikumbh*, Nilesh I. Patil, Swapnil D. Phalak, Sandip S. Chaudhari and Tarannum R. Sayyad
The present work explains the development and validation of a simple and reliable RP-HPLC method for the quantitative determination of Ramipril (RMP). Chromatography was carried out by reversed phase technique on a Fortis C18 (100 mm × 4.6 mm; 2.5 μm particle size). The optimized mobile phase was consisted of methanol and citric acid sodium citrate buffer solution (50:50 v/v) having pH 3.0. The retention times were 3.645 min for RMP. The detection was carried out at 270 nm and a column temperature of 25°C. The method was evaluated for the various validation parameters, such as linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and sample stability. The proposed method was validated and successfully applied for the analysis of pharmaceutical formulations and laboratory prepared mixture containing Ramipril respectively. .. View more»