Mohamed V Hospital, Microbiology Laboratory, Tangier, Morocco
Nadira Mourabit, belonging to the Biotechnology Research and Engineering of Biomolecules Team (ERBGB), interested in the field of Science and Technology, Faculty of Science and Technology, BP 416, Tangier, Morocco
Research Article
Phenotypic and Molecular Study of Carbapenemase-Producing Enterobacteriaceae in a Regional Hospital in Northern Morocco
Author(s): Younes Mahrach, Nadira Mourabit, Abdelhay Arakrak, Mohammed Bakkali and Amin Laglaoui*
Objective: This study aims to assess the prevalence of clinical isolates of carbapenemase-producing Enterobacteriaceae (CPE), and to characterize the types of carbapenemases produced among the strains isolated from different samples in patients hospitalized at the regional hospital in Tangier in the North of Morocco over 12 month period (January to December 2015).
Methods: A total of 367 isolates of Enterobacteriaceae were collected from inpatients, antimicrobial susceptibility of the isolates was determined, and Enterobacteriaceae isolates with reduced susceptibility to carbapenems were investigated by phenotypic study using a range of techniques including antibiotic susceptibility testing, modified Hodge test and the double-disk synergy method. Expression of the genes encoding carbapenemases measured using PCR.
Results:.. View more»