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Nader Hasanzadeh
Department of Plant Pathology, Faculty of Agriculture and Food Industries, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
Publications
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Research Article
Identification and Characterization of Bacterial Agents Causing Moderate Damage and Souring of the Fig Fruits
Author(s): Seyedeh Asiyeh Mousavi*, Nader Hasanzadeh and Parisa Abdollahi
In order to determine the factors of decay and sourness of Fig fruits, in the summer of 2016-17, 60 leaf, fruit and stem samples from different regions of Tehran, Varamin (Qal'e No), Mazandaran (Amol, Noor and Sari), Lorestan and a branch of Fig fruit sample from Italy were collected. The samples were surface disinfected with 70% alcohol, washed and macerated in DH2O and one loopful of each suspension was cultured on NA and KB media. The dominant colonies from each culture were further selected and purified. The pathogenicity tests were fulfilled by Hypersensitivity reaction on geranium leaves and artificially by Fig fruit inoculation. Thirty isolates were considered as pathogenic bacteria and key phenotypic traits were performed. This was confirmed by 16S rRNA gene PCR assay using a pair of universal P1/P6 primer. The amplified PCR products were sequenced by M.. View more»