Journal of Blood Disorders & Transfusion

Department of Materials Science and Engineering, University of Connecticut, Storrs, CT, USA

Publications

• Research Article   
  Morphological Characteristics of Echinocytes: Novel Quantification of Spicule Geometry Using Scanning Electron Microscopy
  Author(s): Samantha Weber-Fishkin, Anna Eligulashvili, Lesley D Frame and Mary D Frame*
  
  Healthy Red Blood Cells (RBCs) are biconcave discs, whose shape is mediated by the components of the erythroid membrane. Pathologic conditions that alter the fluidity of the membrane bilayer result in less deformable cells and, often, abnormal morphologies. Echinocytes are one such morphology characterized by dispersed spicules on the RBC surface. Our objectives are (1) to quantify the well-established qualitative descriptions of stage I, II, and III echinocytes, (2) to determine whether spicule size and symmetry are maintained within a stage of echinocytes, and (3) to determine whether disrupting specific cytoskeletal and transmembrane proteins will result in specific spicule morphologies in cold stored blood. RBCs obtained from healthy human donors were analyzed using optical brightfield and Scanning Electron Microscopy (SEM) imaging. Topology and topography measurements wer.. View more»
  

  DOI: 10.4172/2155-9864.22.13.518

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