Journal of Thrombosis and Circulation: Open Access

Department of Medicine, Metabolic Centre, Karolinska Institutet, Karolinska Universitetssjukhuset, Huddinge, Sweden

Publications

• Research Article   
  An E-cigarette Aerosol Generation, Animal Exposure and Toxicants Quantification System to Characterize in vivo Nicotine Kinetics in Arterial and Venous Blood
  Author(s): Jushan Zhang, Mo Xue*, Rong Pan, Yujie Zhu, Zhongyang Zhang, Haoxiang Cheng, Johan L M Bjorkegren, Jia Chen, Zhiqiang Shi* and Ke Hao*
  
  The increasing e-cigarette use worldwide presents an urgent need to characterize their nicotine delivery property, brain stimulation and potential long-term health effects. We constructed an end-to-end system enabling combustible- cigarette (c-cigarette) and e-cigarette aerosol generation, animal exposure, and effect assessment. The system consists of (1) a 10-channel aerosol generator resembling human smoking/vaping scenarios, (2) nose-only and whole- body exposure chambers suitable for long or short-duration studies, (3) a lab protocol for animal exposure and collecting arterial and venous blood <1 minute after the exposure, and (4) chromatograph and mass spectrometry to quantify nicotine concentrations in aerosol and biospecimens. We applied the system in a proof-of-principle study characterizing in vivo nicotine delivery after e-cigarette aerosol inhalation. Groups of .. View more»
  

  DOI: 10.35248/2572-9462.22.8.198.

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