Journal of Plant Pathology & Microbiology

Department of Entomology, and Plant Pathology, University of the Philippines Los Baños, Laguna, Philippines

Publications

• Research Article   
  Development of Molecular based Method for Specific Detection of Begomovirus Species Causing Tomato Leaf Curl in the Philippines
  Author(s): Jeremy Roy Augustus I. Gomez*
  
  Tomato is one of the most important vegetable crops due to its economic and nutritional value but due to a disease caused by a Begomovirus, tomato production declined over the years. Begomoviruses causing tomato yellow leaf curl or tomato leaf curl affect tomato production in many tropical and subtropical areas of the world. Limited detection on these plant viruses made them so difficult to detect and control. The study aims to develop molecular based method for specific detection of ToLCPV and ToLCCeV. This was done by: 1) Validating whether the published primers could anneal to a specific Begomovirus species using multiple sequence alignment, 2) Designing a primers for specific detection of ToLCPV or ToLCCeV and optimized by PCR, 3) Validating the designed primer for specific detection of ToLCPV and ToLCCeV using field collected samples, 4) To identify RFL.. View more»
  

  DOI: DOI:10.35248/2157-7471.23.14.684

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