Gene Technology

Department of Molecular, Cell and Systems Biology, UC Riverside 900 University Ave, Riverside CA 92521, USA

Biography

Dr Hui Dai is a renowned researcher and author in the field of molecular in UC Riverside 900 University Ave, USA. Now he is working as a research investigator at MD Anderson cancer centre 2000.

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Publications

• Review   
  Strategies and Best Practice in Cloning Small RNAs
  Author(s): Hui Dai and Weifeng Gu*
  
  High-throughput sequencing has become a standard and powerful tool for analyzing nucleic acids primarily due to its sensitivity and convenience. Small RNAs play important roles in regulating cellular and viral genes. The conventional methods for small RNA analyses are tedious and often lack accuracy, specificity and sensitivity for many small RNA species. Therefore, high-throughput sequencing becomes an indispensable tool for analyzing small RNAs. However, it is challenging to generate a reliable and representative small RNA library for high-throughput sequencing since small RNAs are usually expressed at extremely low levels and often contain modifications which affect library construction, usually causing biased readouts. This review compares various strategies for generating small RNA libraries of high quality and reliability, and provides recommendations on best practice in prepari.. View more»
  

  DOI: 10.35248/2329-6682.20.9.151

  Abstract HTML PDF