Journal of Carcinogenesis & Mutagenesis

Department of Applied Life Science, Sojo University, Kumamoto, Japan

Publications

• Research Article   
  Efficacy of Drug-Free Cationic Hybrid Liposomes Composed of L-α Dimyristoylphosphatidylcholine in Targeting Negatively Charged Cholangiocarcinoma Cell Membranes: An In Vitro and In Vivo Study
  Author(s): Hideaki Ichihara*, Hiromitsu Takaki, Muneaki Motomura and Yoko Matsumoto
  
  Cationic Hybrid Liposomes (CL) composed of 87 mol% Dimyristoylphosphatidylcholine (DMPC), 5 mol% Polyoxyethylene (21) Dodecyl Ether (C12(EO)21, also referred to as (HL21), and 8 mol% O,O’-Ditetradecanoyl-N-(α– Trimethylammonioacetyl) Diethanolamine Chloride (2C14ECl) were prepared by sonication. A clear solution of CL with a hydrodynamic diameter of 100 nm was maintained for four weeks. Cholangiocarcinoma cells exhibited a lower zeta potential than normal bile duct cells due to higher levels of negatively charged phosphatidylserine and Ganglioside 1 (GM1). Time-dependent immediate fusion of CL with cholangiocarcinoma cell membranes was confirmed using confocal microscopy. The 50% inhibitory concentration values of CL for the growth of human cholangiocarcinoma cells were markedly lower than those of DMPC and HL21 liposomes... View more»
  

  DOI: 10.35248/2157-2518.2.15.460

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