Department of Health Education and Behavioral Sciences, University of Gondar, Gondar, Ethiopia
Research Article
On-Station and Field Evaluation of Inactivated Fowl Cholera Vaccine Produced from Local Pasteurella multocida Isolates, Ethiopia
Author(s): Zewde Tariku Wolde*, Gezahegn Mamo, Esayas Gelaye, Takele Abayneh and Jaleta Shuka
Vaccination is one of the common preventive measures of fowl cholera. On-station and field study was carried out from November 2017 to April 2018 to evaluate the safety, immunogenicity and protective efficacy of inactivated fowl cholera vaccine developed from local Pasteurella multocida isolate at national veterinary institute, Ethiopia. A total of 60 chickens (8 weeks old; bovan brown breed) randomly divided into 3 groups, each 20 chick, were used for onstation study. The first and second groups were vaccinated twice (three weeks apart) with 1 ml and 0.5 ml of inactivated fowl cholera vaccine containing 2.5 × 108 cfu/ml, respectively both through the IM route. The third group was kept as unvaccinated control. Sera were collected from all groups at day 0, 14, 21, 28 and 35 and kept at -20°C until analysis using Indirect Haemagglutination Test (IHT). All g.. View more»