Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Dr. Frouzandeh Mahjoubi is an Associate professor in the Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. His study interests major on cancer genetics. He published 10 articles in many journals and the articles are informative and got good citations.
Research Article
Comparison RNA Expression Level of HER2 (By QRT-PCR) With Protein Expression Level (Immunohistochemistry) and Investigation HER 2 Expression Level with Clinicopathological Features
Author(s): Samira Shabani, Tayebeh Majidi Zadeh, Ameneh Tavakoli Koudehi and Frouzandeh Mahjoubi*
Background: Evaluation HER2 (human epidermal growth factor receptor 2) status is considered as a standard
practice in breast cancer clinical management. There are different methods for evaluation HER2 status, but currently
the routine method for assessment of HER2 status is Immunohistochemistry (IHC) .The aim of the this study was to
compare the results obtained by IHC and Quantitative Real Time PCR methods in determination of HER2 status to
specify whether QRT- PCR can use as supplementary method in breast cancer or not.
Methods: In this regard, 48 fresh tissues from patients with breast tumor were studied. IHC, qRT- PCR technique
was done in every speciman. IHC was done with DAKO HercepTest and QRT- PCR method was performed with
TaqMan probes and primers in lightcyclerTMsystem (Corbett Real Time Thermal cycler).
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