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Ernesto Mendoza
Department of Molecular Therapy, National Autonomous University of Mexico, Mexico City, Mexico
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Comparison between Two Extraction Methods of Plant Lectins from Ruta graveolens L. and their Partial Characterization
Author(s): Amairany Torres Alvarez, Laura Flores Garcíaa, Ernesto Mendoza* and Catalina Machuca Rodrígueza
Background: Ruta graveolens L. is a plant wich has several uses among different communities, such as an anti- inflammatory and antitumor, related to the plant lectins available in their different tissues. Methods: Extraction of these molecules is achieved through diverse extraction methods, without knowledge about which one is better to preserve their native structure. In this study two well-documented methods are compared. Results: Lectin-enriched extracts were obtained out of different R. graveolens L. organs, and those were purified trough NaCl salt extraction and Tris-HCl extraction methods. Additionally, several eluents were used in the affinity chromatography method. Conclusion: Lectin-like proteins with affinity to several carbohydrates wer.. View more»