Journal of Plant Pathology & Microbiology

School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Mongolia

Publications

• Research Article   
  Gene Transformation of Alfalfa Plants (Medicago varia L.) Domestic Cultivar ‘Burgaltai’ for Increasing Leaf Size
  Author(s): Uuganzaya Myagmarjav*, Enkhchimeg Vanjildorj and Altantsetseg Khajidsuren
  
  ‘Burgaltai’ cultivar of alfalfa is the most important forage in Mongolia and its yield had decreasing last decades due to cross pollination. This study was conducted for increasing leaf size of ‘Burgaltai’ cultivar through Agrobacterium mediated genetic transformation transferring growth regulating factor AtGRF2 gene of Arabidopsis thaliana. In vitro regeneration was derived from seven day old hypocotyl and cotyledon explants for callus induction. Induced calli were used for shoot induction and proliferation. Proliferated shoots carried to root induction medium. The best calli induction medium was MSB5 (Murashige Skoog’s medium with Gamborg’s vitamin) medium supplemented with 2 mg/L 2,4-D, 0.2 mg/L BAP and 1 mg/L NAA, shoot induction and proliferation media from calli were MSB5 medium supplemented with .. View more»
  

  DOI: 10.35248/2157-7471.21.12.578

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