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Attia FM
Department of Plant Pathology, Faculty of Agriculture, Cairo University, Egypt
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Research
A First Record of Exserohilum rostratum as a New Pathogen Causing Bean Blight in Egypt.
Author(s): Farag MF* and Attia FM
Seedling blight of bean (Phaseolus vulgaris L.) was recorded in bean fields at five different localities in Beni Sweif Governorate, Egypt. Symptoms appeared as green dark to purplish-brown spots, with brown margins. The affected plant leaves were collected for mycological analysis. Percentage of disease incidence were 30%, 25%, 22%, 15% and 35% in El-Wasta, Nasser, Beni Sweif, Sumosta and Beba counties respectively. Leaf samples were surface sterilized and cultured on potato dextrose agar. The growing fungi were identified on morphological as well as on molecular basis. Microscopic examination revealed that the isolated organisms have the same characteristics of Exserohilum rostratum (Drechsler) Leonard & Suggs. Among the 30 fungal isolates collected from the five bean plantations, a representative isolate was grown for DNA extraction, PCR and rDNA sequencing. Un.. View more»