Global Journal of Biology, Agriculture & Health Sciences

Department of Pathology, Miyazaki University School of Medicine, Kiyotakecho, Miyazaki, Japan

Publications

• Research   
  Inflammatory Response Induced in Pulmonary Embolic Lung: Evaluation Using a Reproducible Murine Pulmonary Embolism Model
  Author(s): Honoka Okabe, Haruka Kato, Momoka Yoshida, Mayu Kotake, Ruriko Tanabe, Yasuki Matano, Masaki Yoshida, Shintaro Nomura, Atsushi Yamashita and Nobuo Nagai*
  
  Background: To assess the pathophysiological response in pulmonary embolism, we established a novel model using a certain volume of relatively small thrombi in mice. Methods: Thrombi with a maximum diameter of 100 μm or 500 μm were administered intravenously under anesthesia, and the survival ratio was evaluated at 4 hours. The thrombus location, hemodynamics and Computed Tomography (CT) angiography was assessed after thrombus administration. In addition, quantification of cytokine mRNAs and immunohistochemical analysis for interleukin (IL)-6 and CD68 as a macrophage marker were also performed in normal and embolized lungs at 4 hours. Results: Mice with 100 μm clots showed a dose-dependent survival between 2.3 μL/g and 3.0 μL/g 4 hours after embolization. The thrombi were located at the peripheral re.. View more»
  

  DOI: 10.35248/2319-5584.22.11.141

  Abstract HTML PDF