Journal of Bacteriology & Parasitology

Department of Science and Technology, Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou, Benin

Publications

• Research Article   
  Involvement of Pyoverdin, Proteolytic and Lipolytic Enzymes in Pseudomonas aeruginosa Biofilm Formation
  Author(s): Yao Paul Attien*, Comoé Koffi Donatien Benié*, Haziz Orou Sina, Wako-Tianwa Alice Tuo, Arthur Zebre, Clément Kouassi Kouassi, Ibrahim Konaté, Lamine Baba Moussa, Adjehi Dadie and Mireille Dosso
  
  The pathogenicity of P. aeruginosa, which is associated to acute and chronic infections, is related to its ability to form biofilms, and produce spoilage determinants such as pyoverdin, proteases, lipases, and toxins. The purpose of this research was to determine the involvement of pyoverdin, proteolytic and lipolytic enzymes in biofilm formation. A total of 33 strains of P. aeruginosa of animal (11), environmental (11) and clinical (11) origin were identified by PCR followed by sequencing. The microplate method was used to quantify biofilm formation. The pyoverdin on medium was determined by spectrofluorometry. The production of protease and lipase was sought respectively on agar with milk and with Rhodamine B on solid Luria-Bertani medium. In decreasing order of importance, the median value of biofilm formed was 1.5 (environmental strains); 1.3 (clinical.. View more»
  

  DOI: 10.35248/2155-9597.23.14.460

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