Journal of Bioequivalence & Bioavailability

AZIDUS Laboratories Limited, Chennai, Tamil Nadu, India

Publications

• Research Article   
  Novel LC-MS/MS Method for Quantification of Semaglutide in Human Plasma and its Application in Pharmacokinetic Studies
  Author(s): Arjun Arumugam, Geetha Lakshmi, Nageswara Rao Thalapaneni* and Srinivas Gopineedu
  
  The objective of the study was to develop a bioanalytical method for the determination of semaglutide in human K3 EDTA plasma. Separation was achieved by reverse phase chromatography, and quantification was done using the LC-MS/MS method. The chromatographic separation was achieved with an phenomenex aeris wide pore XB-C8 (100 × 4.6 mm) 3.6 μm column thermostated at 40°C with a mobile phase containing gradient program with Pump A: (Acetonitrile: Methanol (50:50) v/v) and Pump B: (0.2% Formic acid in Milli Q Water (v/v)). The flow rate was maintained at 0.800 ml/min, and the injection volume was found to be 5 μl. The detection was done by using high-performance liquid chromatography coupled with tandem mass spectrometry. The retention time was found to be 5.40 ± 0.80 min for the analyte and 5.40 ± 0.80 min for the internal standard. The calibrat.. View more»
  

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