Journal of Microbial & Biochemical Technology

Infection, Prevention and Control Department, 57357 Children Cancer Hospital, El-Saida Zenab, Cairo, Egypt

Publications

• Research   
  rpoB, katG and inhA Genes: The Mutations Associated with Resistance to Rifampicin and Isoniazid in Egyptian Mycobacterium tuberculosis Clinical Isolates
  Author(s): Amal M Hosny*, Hala M Abu Shady and Ayman K El Essawy
  
  In response to the huge number of people who die yearly due tuberculosis and the emergence of multidrug resistant (MDR) M. tuberculosis, accurate and rapid detection of this resistance can improve the situation. Relapsed patients in the current work represented significant percentages among rifampicin and isoniazid resistant isolates compared to other risk factors. Two molecular techniques (Genotype MTBDRplus assay and specific gene sequencing were used to detect associated mutations in TB drug resistant isolates. The genotypic profile of Multi-drug resistant (MDR) isolates showed missing of katG wild type 1 (WT1) band. Eighty percent of isoniazid mono-resistant isolates, showed katG MUT1, 20% showed katG MUT1 and inhA MUT1, 20% showed only inhA MUT1. The molecular techniques partly predicted the level of antibiotic resistance associated with katG and/or inhA gene mutations (for isoni.. View more»
  

  DOI: 10.35248/1948-5948.20.12.428

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