Biology Department, College of Sciences, Kerbala University, Iraq
Research Article
Dietary Supplementation of Bacillus subtilis as Probiotic affects Haemato-immunological Parameters of Common Carp (Cyprinus carpio)
Author(s): Ali A. Abid Al-Hisnawi* and Doaa Ali Beiwi
The current study was conducted to investigate the influence of Bacillus subtilis feed additive on hematological
parameters, blood serum parameters and the levels of cytokines, including Transforming growth factor beta (TGF-β)
and Interleukin 1 beta (IL-1β), of common carp. The probiotic was isolated from local soils using PCR approach
for amplification of 16S rDNA and added to the diet in a dose of ~107 CFU g−1. The feeding trial was carried
out for six weeks. A total of 64 fish (77.2 ± 0.86 g) were randomly divided into two groups: control group (basal
diet) and experimental group. Compared to the control group, the carp fed probiotic supplemented diet displayed
a significant elevation in mean cell haemoglobin concentration (P <0.05). The other haematology parameters
were not significantl.. View more»