Journal of Stem Cell Research & Therapy

Biologics Technology Research Laboratories II, Biologics Division, Daiichi Sankyo Co., Ltd., 2716-1, Aza Kurakake, Oaza Akaiwa, Chiyoda-machi, Oura-gun, Gunma 370-0503, Japan

Publications

• Research Article   
  Acetyl Amino Acids Prevent Caspase 9-mediated Apoptosis Induced by Octanoate in Mesenchymal Stem Cells
  Author(s): Osamu Kamida*, Shuo-Hao Huang, Akira Kawaguchi and Takahisa Shimizu
  
  Raw materials affect quality of cell therapy products. Although human serum albumin products as pharmaceuticals have been reported to improve cell quality, Sodium Octanoate (SO), which is known to damage cells, is contains as an additive of human serum albumin drug products. We confirmed SO concentration-dependent decrease in cell viability and increase in apoptosis in Mesenchymal Stem Cells (MSCs). Furthermore, we detected decrease of mitochondrial transmembrane potential difference and caspase 9 activation in a dose dependent manner of SO. N-Acetyl-L-Cysteine (NAC) and N-Acetyl- L-Tryptophan (NAT) were investigated for their protective effects towards SO-induced cell death and apoptosis due to their antioxidant properties. It was identified that the SO dependent apoptosis, caspase 9 activation and cell death were suppressed by NAC or NAT. These results suggest that the SO dependent .. View more»
  

  DOI: 10.35248/2157-7633.23.13.602

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