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Akihiro Ohnishi
Department of Fermentation Science, Tokyo University of Agriculture, Tokyo, Japan
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Research Article
Genomic Analysis of Tepidimicrobium xylanilyticum: Another Key to Lactate Metabolic Pathway in Thermophilic Anaerobic Digestion
Author(s): Hou-Chia Tseng*, Minenosuke Matsutani, Naoshi Fujimoto and Akihiro Ohnishi
Tepidimicrobium xylanilyticum is a thermophilic lactate-utilising bacterium that produces H2, CO2, and acetate in pure culture using lactate as the sole carbon source. This study aimed to clarify the Thermophilic Lactate Metabolic (TLM) pathway and related genes in T. xylanilyticum participating in lactate metabolism during Thermophilic Anaerobic Digestion (TAD). De novo genome sequencing was used to determine the genomic characteristics of T. xylanilyticum. The annotations and TLM pathways of T. xylanilyticumwere predicted using DFAST and GhostKOALA. Comparative genomic analysis between the isolate EN5CB1 and type strain DSM 23310 of T. xylanilyticum was performed using CD-HIT and CLUSTALW. The genome size of T. xylanilyticum was approximately 2.9-3.1 Mbp with 33.2%-33.3% average GC-conten.. View more»