Agrotechnology

Abstract

Selection of Rhizobacteria to Control Twisted Disease of Shallot

Dina Istiqomah*, Ayu Lestiyani, Arif Wibowo, Tri Joko and Siti Subandiyah

The purpose of this study is to find out the type of antagonistic rhizobacteria and their capability in F. acutatum growth suppression on in vitro and in vivo condition. The study was conducted by selecting of rhizobacteria isolated from Shallot’s rhizosphere which was taken from 4 regions; they were Bantul district (Yogyakarta), Nganjuk (East Java), Cirebon (West Java) and Brebes (Central Java) district. Characterization was taken based on physiology and biochemist, PCR based on 16S rRNA and Rep-PCR (BOX-PCR and ERIC-PCR). The in vivo test was carried out by factorial experiment with 2 trials using completelly randomized design i.e., 1) soaking shallot seeds into rhizobacteria suspension for 1 hour before planting and 2) watering plants with rhizobacteria suspension in once 2 weeks. Observation variables consists of incubation period, disease incidence, plant high, fresh and dry weight of plant and total Fusarium population.

The results showed that there were some beneficial rhizobacteria from shallot’s rhizosphere which could be used to control twisted disease on shallot, which consisted of Bulkholderia and Bacillus. The isolate BrSG.3 closely related to Burkholderia seminalis, isolate BrSG.5 and Bp25.6 closely related to Bacillus amyloliquofaciens, isolate BrSM.4 closely related to Burkholderia cepacia strain NTUIOB TPC6, isolate Bp25.2 closely related to Bacillus methylothrophicus and isolate Bp25.7 closely related to Bacillus subtilis. The bacterial isolates which are potential for biological control and increased of plant growth were Bacillus spp. isolates.

Published Date: 2024-07-29; Received Date: 2019-12-18