Journal of Food Processing & Technology

Abstract

Identification and Characterization of Caffeine Degrading Microbes from Coffee Husk for Production of Liquid Bio Fertilizer and Process Optimization by Using Rhizobium Bacteria

Zeynu Shamil*

Bioremediation being a problem solver for many different environmental and other major hazards, they play an important and cost effective role in the decaffeination process. Within this experimental work eliminate caffeine from coffee husk then we take Rhizobium bacterial strain from Ethiopia bio diversity institute. We observe that could grow on the medium supplemented with 2.5g/L of caffeine i.e., could effectively degrade 2.5 g/L of caffeine in the liquid media as a sole source of carbon and nitrogen. Morphological and biochemical characteristics were maintained for re-affirmation the organism as Rhizobium . The degradation of caffeine capability was authenticated by growth curve. And secondly we observed that the potency of caffeine degradation caffeine degrading bacteria was maintained by standard medium with and without supplement of 2.5g/l of caffeine then we quantify no. of cell grow within the medium then finally we optimized the factors by using experimental design software. The result we obtained temp; 35°C, pH 7.07 incubation time 36 hr from those optimal condition obtained maximum cell growth in number was 3.41995E+008 and 5.0707E+008 with and without caffeine respectively.

Published Date: 2024-09-13; Received Date: 2020-11-23