Biology and Medicine

Abstract

Comparison between Two Extraction Methods of Plant Lectins from Ruta graveolens L. and their Partial Characterization

Amairany Torres Alvarez, Laura Flores Garcíaa, Ernesto Mendoza* and Catalina Machuca Rodrígueza

Background: Ruta graveolens L. is a plant wich has several uses among different communities, such as an anti- inflammatory and antitumor, related to the plant lectins available in their different tissues.

Methods: Extraction of these molecules is achieved through diverse extraction methods, without knowledge about which one is better to preserve their native structure. In this study two well-documented methods are compared.

Results: Lectin-enriched extracts were obtained out of different R. graveolens L. organs, and those were purified trough NaCl salt extraction and Tris-HCl extraction methods. Additionally, several eluents were used in the affinity chromatography method.

Conclusion: Lectin-like proteins with affinity to several carbohydrates were found. The NaCl extraction method was more efficient at extracting lectin-like proteins than the Tris-HCl method, comparing to the total-protein extract. These results suggest that Tris-HCl buffer is more efficient to extract other proteins.

Published Date: 2024-02-07; Received Date: 2024-01-06