Journal of Stem Cell Research & Therapy

Abstract

Acetyl Amino Acids Prevent Caspase 9-mediated Apoptosis Induced by Octanoate in Mesenchymal Stem Cells

Osamu Kamida*, Shuo-Hao Huang, Akira Kawaguchi and Takahisa Shimizu

Raw materials affect quality of cell therapy products. Although human serum albumin products as pharmaceuticals have been reported to improve cell quality, Sodium Octanoate (SO), which is known to damage cells, is contains as an additive of human serum albumin drug products. We confirmed SO concentration-dependent decrease in cell viability and increase in apoptosis in Mesenchymal Stem Cells (MSCs). Furthermore, we detected decrease of mitochondrial transmembrane potential difference and caspase 9 activation in a dose dependent manner of SO. N-Acetyl-L-Cysteine (NAC) and N-Acetyl- L-Tryptophan (NAT) were investigated for their protective effects towards SO-induced cell death and apoptosis due to their antioxidant properties. It was identified that the SO dependent apoptosis, caspase 9 activation and cell death were suppressed by NAC or NAT. These results suggest that the SO dependent cell death on MSCs is likely due to mitochondrial dysfunction by Reactive Oxygen Species (ROS) generation or cytoplasmic release of cytochrome c, and the acetyl amino acids play a critical role in inhibiting the SO dependent cytotoxicity on MSCs. Our research indicated that inhibitory effects of acetyl amino acids may contribute to the process development in cell therapy field.

Published Date: 2023-08-18; Received Date: 2023-06-02