Journal of Nanomedicine & Nanotechnology

ZnO nanoparticles a useful candidate to arrest the cancer cells of human hepatocellular carcinoma, human breast adenocarcinoma and their antibacterial activity

3^rd International Conference on Nanotek & Expo

December 02-04, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Rizwan Wahab

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Liver and breast cancer are the most shattering disease, which affects the major organs of the body. The nanoparticle of ZnO has a property to the entry into the body through any of the possible routes. Therefore, the aim of the present study was to investigate the activity against HepG2 and MCF-7 Cells, with zinc oxide nanoparticles (ZnO-NPs). The NPs (~13?2 nm) were prepared via non protonated chemical route and characterized with the standardtechniques such as X-ray diffraction spectroscopy used to know the crystalline property of NPs whereas, the morphology, functional and optical analysis were performed with FE-SEM, TEM, FTIR, UV visible spectroscopy respectively.The study shows that the treatments with NPs are very effective on HepG2 and MCF-7 cancer cells at dose dependant manner. The MTT assays revealed the concentration dependent cytotoxic effects of NPs in range of 2.5-100 μg/ml. HepG2 and MCF-7 cells were exposed to ZnO-NPs and it exhibited significant reduction (5% and 4%; p<0.05) at a very low concentration 25 μg/ml and justified with FACS data. Thus, the reduction in cell viability with NPs induces cytotoxicity in cultured cells. Quantitative RT-PCR demonstrates, exposure of HepG2 cells to ZnO-NPs and the level of mRNA expressions (Bax, P53, and Caspase-3) were significantly up-regulated, whereas the anti-apoptotic gene (Bcl-2) were down-regulated. The NPs were also tested against five pathogenic ( E.coli, S.aureus, P. aeruginosa,B. subtilis and S.acidaminiphila ) bacteria and compared with salt of ZnO via disk diffusion method and it?s found that NPs achieved good inhibition of microbial strains.

Biography :

Rizwan Wahab is working as an Assistant Professor in the department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. He worked for his doctorate degree in the department of Chemical Engineering, Chonbuk National University, Jeonju South Korea in 2008. He has published 40 international papers in reputed Journals on the topic of fabrication of nanostructures and their applications such as microbiology, cell biology, DNA damage, biosensing, photocatalysis etc. He worked on several projects such as novel bio-devices by Nano-bio fusion technology and their applications. His current research interest is applications of nanostructures for the anticancer and antimicrobial study