Journal of Microbial & Biochemical Technology

Whole genome sequence based genotypic-phenotypic comparisons of Mycobacterium tuberculosis causing MDR-TB and XDR-TB in Thailand

16^th International Pharmaceutical Microbiology and Biotechnology Conference

May 21-22, 2018 | Vienna, Austria

Kiatichai Faksri

Khon Kaen University, Thailand
Research and Diagnostic Center for Emerging Infectious
Diseases - Khon Kaen University, Thailand

Keynote: J Microb Biochem Technol

Abstract:

Drug resistant tuberculosis (TB) is a major public health problem. The information regarding genotypic-phenotypic association of anti-TB drugs were limited, especially for the second line drugs. The aim of this study is to compare the concordant rate between whole genome sequence (WGS) based genotypic and phenotypic drug susceptibility testing of Mycobacterium tuberculosis (Mtb) (n=293). Drug susceptibility test using standard proportional method (agar based test) and whole genome sequencing (WGS) using Illumina platform of Mtb causing drug sensitive (n=51), mono-resistant (n=38), multi-drug resistant (MDR) (n=96), MDR with fluoroquinolone resistant (n=84) and extensively-drug resistant (n=23) TB in Thailand during 19982016 were performed. WGS analysis was performed using genetic mutation databases from PhyResSE and TB-Profiler program. It was found that the concordant rate between PhyResSE based genotype and phenotype of the first line drugs and second line drugs were 91.4% and 77.8% respectively. The concordant rate between TB-profiler and phenotype of the first line drugs and second line drugs were 91.96% and 80.65% respectively. We found that particular drug in fluoroquinolone group were not homogeneously concordant to phenotypic results, e.g. Ofloxacin 90.3% vs Gatifloxacin was 56.6%. This study reported that the drug resistance mutation databases, especially for the second line drugs need to be improved. TB-Profiler database provide higher performance for detection of drug resistant TB for both the first and second line drugs. The mutations in gyrA and gyrB were not homogeneously associated to particular drug in fluoroquinolone groups.

Biography :

Kiatichai Faksri has graduated PhD in Medical Microbiology from Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand and Postdoctoral studies from the National University of Singapore (NUS) for doing researches related to whole genome sequence (WGS) analysis of M.tuberculosis. His research focuses on several aspects of TB such as drug resistant TB and molecular diagnosis of TB. In the last four years, 2014-2017, he has published 25 research articles in which 15 of those he is the first and/or corresponding author.
Email:kiatichai@kku.ac.th