Agrotechnology

Variation in the antagonistic potential of Trichoderma spp. against Sclerotium rolfsii in vitro

International Conference on Agricultural & Horticultural Sciences

September 14-15, 2012 Hyderabad International Convention Centre, India

Swathi B and Patibanda A. K

Posters: Agrotechnol

Abstract:

Isolate Trichoderma harzianum Th 4 and T. virens Tv 5 were found effective against Sclerotium rolfsii, Chickpea collar rot patho - gen, when twenty one sympatric Trichoderma isolates were assessed for their antagonistic potential in vitro using dual culture method. The test Trichoderma isolates were categorized based on their interactions in dual culture through qualitative param - eters such as formation of zone of inhibition, lysis and over growth. Successful antagonism resulted in penetration of Tricho - derma in to S. rolfsii zone causing lysis of S. rolfsii hyphae by occupying the thick strands and forming dark green spore pustules. Comparisons were made between T. harzianum Th 4 with faster radial growth in monoculture, and simultaneous lysis and over growth on S. rolfsii and T. virens Tv 5 isolate with slow radial growth in monoculture and over growth followed by lysis of S. rolfsii. Volatile metabolites of isolate Tv 5 were more inhibitory to S. rolfsii growth (54.6% inhibition) compared to Th 4 (16.3% inhibition) while non volatile metabolites of Th 4 (100% inhibition at 60% concentration) were more effective compared to Tv 5 (69.3% at 80% concentration) against S. rolfsii.

Biography :

B. Swathi has completed M. Sc (Agriculture) in Plant Pathology and presently pursuing doctoral programme in Dept of Plant Pathology from Acharya N.G. Ranga Agricultural University, Agriculture College, Bapatla, Andhra Pradesh.