Pharmaceutica Analytica Acta

Two-dimensional microscale sample pretreatment technique for MS/MS comprehensive analysis of complex samples

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Donghao Li, Ma Biao and Jinhua Zhao

Yanbian University, China

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

A novel integrated on-line two-dimensional microscale carbon fiber columns coupled with MS/MS system (2DμCF-MS/MS) was firstly realized and it was applied in the comprehensive analysis of complex samples containing various polarity components. We used two different surface carbon fibers as packing material to form an integrated 2Dμ CF column. The non-polar chemicals are retained on the carbon fiber (CF) column and polar and medium-polar chemicals are passed through CF column and absorbed on the second column which is packed with activated carbon fiber (ACF). The three classes of chemicals are subsequently separated into three fractions by optimized mobile phase. Using the online combination of integrated CF columns with MS/MS system, the chemicals were simultaneously ionized one by one group and transferred into MS system for detection. As a result, the three groups are covering whole chemicals in the extract and sensitivity is also significantly improved by reducing interferences and ionization suppression effect. The system has satisfactory accuracy (55% to ~92%), LOD (0.15 ng gâ??1 to ~25 ng gâ??1), reproducibility (RSD=0.23% to ~21.65%). The proposed method has been applied to comprehensive analyze Schisandrae chinensis, it could high throughput determine diverse polarity compounds including organic acids, lignans and flavonoids in a single injection with less than 7 minutes, low pressure separation and small amount of solvent. So the present study shows that the technique is a high throughput, rapid and versatile analytical method which allows a wide range of applications in comprehensive analysis of complex samples and biological metabolic study.

Biography :

Donghao Li has completed his PhD from University of Konkuk University, Korea. He is an analytical Chemist, Professor and Director of a “Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, China. He has published more than 70 papers in reputed journals and is serving as an Editorial Board Member of repute.

Email: dhli@ybu.edu.cn