Journal of Nanomedicine & Nanotechnology

Two Dimensional Fluorescence Difference Spectroscopy (2D FDS) to characterize nanoparticles and their interactions

8^th World Medical Nanotechnology Congress & Expo

June 08-09, 2016 Dallas, USA

Miranda N Hurst and Robert K DeLong

Kansas State University, USA

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Two dimensional fluorescence difference spectroscopy (2D FDS) is an innovative technique to detect nanoparticles and validate interaction upon surface functionalization and biomolecule loading. 2D FDS detects emission while scanning multiple excitation wavelengths, generating a contour plot with fluorescent intensity reflected as color per an excitation and emission intersect, also called a spectral signature. Initially metal oxide nanoparticles of various compositions were compared, where 2D FDS revealed a unique spectral signature per material composition. To gauge detection of surface functionalization, zinc oxide nanoparticles (ZnO NP) were loaded with one of three polymers: glycol chitosan, polyacrylic acid (PAA), and methoxy polyethylene glycol (mPEG). 2D FDS revealed a shift in the spectral signature in the presence of the polymer. ZnO NP was loaded with one of three RNAs: Torula Yeast RNA (TYRNA), polyinosinic: polycytidylic acid (pIC), and splice switching oligonucleotide (SSO), to determine the efficacy of 2D FDS in identifying biomolecular interactions. The greatest spectral shift was observed for pIC, followed by SSO and TYRNA. Extending this technique from nanoparticles to proteins, Ras binding domain (RBD) is a derivative of B-Raf protein capable of binding Ras and thus a potential cancer therapeutic. RBD was shown to give a spectral signature, where a shift in the optimal excitation and emission intersect elucidates protein conformation. In the presence of ZnO NP and iron oxide nanoparticle (Fe2O3 NP), a shift was observed for both particles. Altogether, these data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions.

Biography :

Miranda N Hurst received her Bachelor of Science in Cell and Molecular Biology from Missouri State University in 2015. She is currently working as a Research Assistant under Dr. Robert Delong at the Nanotechnology Innovation Center of Kansas State (NICKS). Her current research focuses on the stabilization and delivery of RNA via nanoparticles into human and mouse cell lines. Her research interests include the relationship between structure and function of RNA and protein in the complex cellular environment, specifically for the development of cancer therapies. She has 2 publications in peer reviewed journals and 1 patent submitted.

Email: mnhurst@ksu.edu