Journal of Aquaculture Research & Development

Tubulin mediates Portunus trituberculatus reovirus infection

4^th International Conference on Fisheries & Aquaculture

November 28-30, 2016 San Antonio, USA

Jing Fang, Dengfeng Li, Ran Xu, Liping Zhang, Lianguo Liu and Annan Guo

Ningbo University, China

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

Reoviruses are widespread and infect a broad range of hosts. To date, no study has been reported on an aquatic reovirus receptor. By using viral overlay protein binding assay (VOPBA), swimming crab Portunus trituberculatus reovirus (SCRV) was found to bind to a protein of approximately 550 kDa. MALDI-TOF MSâ??MS analysis revealed that the protein shares the closest homology with β-tubulin. Mouse membrane proteins were tested by western blot with antibodies against the SCRVbinding protein and mouse tubulin, and uniform positive bands were obtained. The results indicated that the SCRV-binding protein was tubulin. The interaction between tubulin and SCRV was further confirmed with co-immunoprecipitation. SCRV infection in vitro could be blocked by a tubulin-specific antibody. The role of tubulin as a major cell surface protein has been reported previously. These findings suggest that tubulin mediates SCRV infection and may function as a receptor for SCRV.

Biography :

Jing Fang is a PhD student at Ocean College of Qinzhou University, Qinzhou, China.

Email: 924113343@qq.com