Journal of Bacteriology & Parasitology

Transcription factor c-Rel plays a role in driving experimental acute and chronic ileitis in rats

7^th World Congress on Microbiology

November 28-29, 2016 Valencia, Spain

Zherebyatiev A S, Kamyshny A M, Kozachuk A S and Kamyshna V A

Zaporozhye State Medical University, Ukraine

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Statement of the Problem: Genetic and environmental factors, including the commensal microbiota, have a crucial role in the development of inflammatory bowel disease (IBD). IBD is also associated with chromosome 2p16, which contains REL, which encodes c-Rel, a subunit of NF-κB. Aberrant activation of the transcription factor NF-κB is associated with acute and chronic intestinal inflammation in rats and plays a key role in cytokine gene regulation, in patients with IBD. c-Rel is required also within cells of the innate immune system for the activation of T cell-dependent as well as innate mechanisms of mucosal inflammation. Aim: The purpose of this study is to investigate the expression of the NF-κB subunit c-Rel in the development of acute and chronic ileitis in rats. Methodology & Theoretical Orientation: Male Wistar rats weighing 200-250 g were housed in standard wire-mesh bottom cages at constant temperature of 25 °C and 12/12 hours light/dark cycles. Acute ileitis was induced in fed rats (n=10) by one subcutaneous injections of indomethacin, an inhibitor of the cyclooxygenase pathway of arachidonic acid (15 mg/kg). Chronic ileitis was induced by two subcutaneous injections of indomethacin (10 mg/kg in 5% freshly prepared NaHCO3 at 37 °C) were administered 24 hours apart. Expression of c-Rel mRNA was determined by real-time reverse-transcription polymerase chain reaction performed using a CFX96� Real-Time PCR Detection Systems (Bio-Rad Laboratories, Inc., USA). GAPDH was used as endogenous control to normalize gene expression data and a relative quantitation value. All statistical analyses were performed using STATISTICA 6.0 software. Results are expressed as mean values±SEM. Findings: The expression of c-Rel was assessed in ileum. Greater expression of c-Rel predominated during chronic ileitis in rats compared to control group (4.8±0.7, P<0.003). Consistent with the pronounced expression during chronic disease, the level of c-Rel expression was also elevated in rats with acute ileitis (4.1±1.1, P<0.02). Conclusion & Significance: In summary, these results suggest that the expression of c-Rel in ileum is essential for initiating intestinal inflammation and may advance our understanding of IBD pathogenesis and that targeting NF-κBc-Rel can be used as a novel molecular approach for the treatment of patients with IBD.

Biography :

Email: Gerya2009@yandex.ru