Journal of Stem Cell Research & Therapy

Tracing and characterizing the development of transplanted female germline stem cells in vivo

9^th Annual Conference on Stem Cell and Regenerative Medicine

September 25-26, 2017 Berlin, Germany

Ji Wu, Changqing Wu, Bo Xu, Xiaoyong Li and Geng Tian

Shanghai Jiao Tong University, China

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Recent evidence has demonstrated the isolation and culture of Female Germline Stem Cells (FGSCs) from adult mice and humans. However, the process and mechanisms of FGSC differentiation in vivo following transplantation have not yet been studied. Here we isolate and characterize FGSCs from a single EGFP-transgenic mouse. Results show FGSCs exhibit a homing ability and begin to differentiate into early-stage oocytes only when they reach the edge of the ovarian cortex. The transplanted FGSCs restore function of premature ovarian failure (gdf-9iCre; PtenloxP/loxP genotype) and generate offspring. Furthermore, results demonstrate that the developmental mechanisms of follicles derived from transplanted FGSCs are similar to that of WT follicles. Weighted gene co-expression network analysis identifies two potential sub-networks and core genes that play a critical role in follicular development. These findings provide a theoretical basis and lay a technology platform for specific or personalized medical treatment of ovarian failure or other ovarian diseases.