Pharmaceutica Analytica Acta

Therapeutic potential of ultraviolet-C: Hematogenous oxidation therapy in septic animal model

International Conference on Bio-Pharmaceuticals, Biosimilars & Pharma Industry

February 28 - March 01, 2019 Osaka, Japan

Gareeballah Osman Adam, Jongsuk Kim, Gi-Beum Kim, Dong-Kwon Yang, Shang-Jin Kim, Jin-Shang Kim and Hyung-Sub Kang

Chonbuk National University, South Korea

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Sepsis mainly characterized by multiple-organ dysfunction. Patients still receive replacement therapy because no radical sepsis treatment. Physical treatment approach including UV blood irradiation was introduced as an alternative treatment for infections since last century. Therefore, UV-C Hematogenous Oxidation Therapy (UV-C HOT) as a physical rather than chemical treatment was investigated in sepsis rabbit model. 0.1 mg/Kg of Lipopolysaccharide (LPS) was used to induce sepsis in rabbits. HOT device was made (Figure.1) UV lamps adjusted at distance of 5-120 mm. The blood was collected in sodium citrate solution, perfused with oxygen for 10 seconds, then UV light with the intensity of 10.290 J/cm2 was irradiated to the blood which passes at a 1.25 ml/min flow rate using the infusion pump in the HOT device. After the HOT was performed, the blood was transfused back to the original rabbit. Blood and biochemical parameters were analyzed using Automatic analyzers. Survival rate, liver, kidney, lipid profile and blood ions were assessed in UV-C HOT treated rabbits. Four rabbits out of ten were died in LPS group while other groups recorded no death. UV-C HOT modulated critical pH, base deficit, disturbed blood gases, hypomagnesemia, hyperlactatemia and concurrent acidosis. In addition, TNFα, nitrite and nitrate were suppressed in response to UV-C HOT. Moreover, UV-C HOT reduces liver and kidney enzymes, improved lipid metabolism and electrolytes homeostasis. Collectively, these results propose that UV-C HOT can reduce mortality, improves magnesium and lactate, possesses antioxidant and anti-inflammatory actions. UV-C HOT performance in ICU for human septic patients can be evaluated and considered.

References

1. Larsen FF, Petersen JA (2017) Novel biomarkers for sepsis: A narrative review. Eur J Intern Med; 45: 46-50.

2. Esposito S, De Simone G, Boccia G, De Caro F, Pagliano P (2017) Sepsis and septic shock: New definitions, new diagnostic and therapeutic approaches. J Glob Antimicrob Resist; 10: 204-212.

3. Yucel G, Zhao Z, El-Battrawy I, Lan H, Lang S, Li X, Buljubasic F, Zimmermann WH, Cyganek L, Utikal J, Ravens U, Wieland J, Borggrefe M, Zhou XB, Akin I (2017) Lipopolysaccharides induced inflammatory responses and electrophysiological dysfunctions in human-induced pluripotent stem cell derived cardiomyocytes. Sci Rep; 7(1): 2935.

Biography :

Gareeballah Osman Adam has published 7 articles in scientific journals. He is a Member of American Society of Pharmacology and Experimental Therapeutics (ASPET).

E-mail: gorba000@gmail.com