Journal of Nanomedicine & Nanotechnology

The nanoparticle protein corona relevance for nanomedicine and toxicology

3^rd International Conference on Nanotek & Expo

December 02-04, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Roland H. Stauber

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

In biological fluids, proteins bind to the surface of nanoparticles forming a coating known as the protein corona, potentially influencing success or failure of nanobiomedical applications. Nanomaterials adsorb biomolecules upon contact with all (biological) environments. Therefore, the biomolecules-coated nanomaterials may need to be considered as ?new materials? compared to the pristine nanomaterials during their manufacturing. Particularly, the so called ?nanoparticle-protein corona? is expectedto influence not only the success and safety of nanobiomedical applicationsbut alsoto critically impact nanotoxicology and nanoecology. As most biological systems are (highly) dynamic, a time-resolved knowledge of particle-specific protein fingerprints is required to understand the coronas? evolution, enabling improved nanotechnological applications without potential adverse side effects. Employing label-free liquid chromatography mass spectrometry, we present not only a qualitative but also a quantitative systematic analysis of the human blood protein corona on nanoparticles varying in distinct physico-chemical features. Our results provide novel insights into the complexity and kinetic evolution of particle-specific protein signatures. Collectively, we demonstrate that already the rapid corona formation is (patho) biologically relevant and provide bioinformatic functional predictors. Combined with comprehensive cell-based (high-throughput) assays, the impact of corona evolution as well as its rational exploitation for advanced nanomaterial with improved safety will be discussed