Journal of Microbial & Biochemical Technology

The high frequency of uropathogenic Escherichia coli strains carrying aerobactin gene isolated from clinical cases

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Reza Ranjbar, Seyedeh Marjan Mojabi, Ali Najafi and Nadia Jahandeh

Baqiyatallah University of Medical Sciences, Iran

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

Objectives: Uropathogenic Escherichia coli (UPEC) are one of the most important etiologic agent of urinary tract infection (UTI). UPEC stains have various types of virulence factors including adhesions, toxins and iron uptake systems. Virulence genes are mainly located on transmissible genetic elements and/or in particular locus on the chromosome called pathogenicity islands (PAI). The aim of this study was to investigate the frequency of uropathogenic E.coli strains carrying aerobactin gene recovered from the patients admitted to Baqiyatallah hospital in Tehran, Iran. Methods: The urine samples were collected from hospitalized patients with UTI admitted to Baqiyatallah hospital, Tehran, Iran from April to October 2014. Standard microbiological and biochemical techniques were used to identify the E. coli strains. Total genomic DNA was extracted using boiling method. Polymerase chain reaction (PCR) using specific primers were used for detection of virulence gene encoding aerobactin among the strains. The PCR amplicons were visualized after electrophoresis and staining with ethidium bromide. Results: Fifty E. coli strains were isolated and included in the study. The results obtained from PCR amplification showed that aerobactin gene was amplified successfully with the final size of 602 bp in all stains. The frequency of uropathogenic E. coli strains harboring aerobactin gene was %88. Conclusion: Our finding indicated that the frequency of uropathogenic E. coli strains carrying aerobactin gene in the study is very high and can pose a threat for spreading of the organism in the hospital.