Journal of Microbial & Biochemical Technology

The comparison of C. coum or C. coum-ciprofloxacin treated biofilms with untreated by 2-DE method

5^th International Conference on Microbial Physiology and Genomics

September 29-30, 2016 London, UK

Morvarid Shafiei, Ahya Abdi-Ali, Azra Saboora, Fereshteh Shahcheraghi, Hojatollah Vali and Kambiz Akbari Noghabi

Pasteur Institute of Iran, Iran
Alzahra University, Iran
McGill University, Canada
National Institute of Genetic Engineering and Biotechnology, Iran

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Biofilm infections with Pseudomonas aeruginosa are the major cause of premature death associated with cystic fibrosis. Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilm-related infections. We previously showed that n-butanolic Cyclamen coum extract in combination with ciprofloxacin removed one and three daysold biofilms. Here, we compared protein expression in P. aeruginosa PAO1 treated and untreated biofilms by 2-DE method. According to 2-DE results, proteins which were involved in alginate biosynthesis, quorum sensing, adaptation, carbon and amino acid metabolism were weakly expressed in n-butanolic C. coum extract ciprofloxacin treated biofilm cells. It seems likely that, combined exposure to both n-butanolic C. coum extract and antimicrobial agents such as ciprofloxacin may offer a novel strategy to control pre-established, persistent P. aeruginosa biofilms and biofilm-related infections.

Biography :

Morvarid Shafiei1is associated with Department of Bacteriology, Pasteur Institute of Iran, Tehran, IR Iran. Morvarid Shafiei has published several papers in reputed journals. Morvarid Shafiei is committed to highest standards of excellence and it proves through the authorship of many books. Morvarid Shafiei research interests include Microbiology, Molecular Biology, Genetics, Agricultural Plant Science.

Email: Dr.shafiei80@gmail.com